Journal: Cell stem cell

Article Title: Broad Distribution of Hepatocyte Proliferation in Liver Homeostasis and Regeneration

doi: 10.1016/j.stem.2019.11.001

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Immunodetection Kit Vector Laboratories Cat# BMK-2202 RNAscope 2.5 HD Reagent Kit-RED Advanced Cell Diagnostics (ACD) Cat# 322350 RNAscope Probe-Mm-Axin2 Advanced Cell Diagnostics (ACD) Cat# 400331 RNAscope Probe-Mm-Tert Advanced Cell Diagnostics (ACD) Cat# 313441 Streptavidin, Alexa Fluor 647 Thermo Fisher Scientific Cat# {"type":"entrez-protein","attrs":{"text":"S21374","term_id":"99986","term_text":"pir||S21374"}} S21374 Streptavidin/Biotin Blocking Kit Vector Laboratories Cat# SP-2002 Experimental Models: Organisms/Strains R26R rb/rb mice Red-Horse et al., 2010 N/A R26R tdRFP/tdRFP mice Luche et al., 2007 N/A R26R ZG/ZG mice Schaub et al., 2018 N/A C57BL/6J mice Jackson Laboratory Stock# 000664 Software and Algorithms Creative Suite Adobe CC 2017 Excel Microsoft Office 16 Fiji Schindelin et al., 2012 N/A Prism GraphPad Version 8.1.2 Other BX51 fluorescence microscope Olympus N/A TCS SP5 confocal microscope Leica N/A VT1000 S vibrating-blade microtome Leica Cat# 14047235613 Open in a separate window KEY RESOURCES TABLE Random lineage tracing provides a representative sample of all hepatocytes Liver homeostasis relies on modest proliferation of hepatocytes in all zones The burden of proliferation in liver regeneration is distributed among hepatocytes Chronic injury reveals differences in hepatocyte proliferation caused by ploidy

Techniques: Transduction, Virus, Plasmid Preparation, Recombinant, Immunohistochemical staining, Histopathology, Immunodetection, Blocking Assay, Software, Fluorescence, Microscopy